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## 1. Understand gracilis muscle anatomy and neurovascular supply
### a) Explain the markings of the pedicle
- Key surface landmarks
- Mark the pubic tubercle (pubic symphysis region) and the medial tibial condyle first — these define the gracilis axis (pubis → medial tibial condyle).
- Palpate and mark the adductor longus tendon/muscle (becomes prominent with thigh abduction/“frog‑leg” or knee extension). The gracilis runs just posterior to the adductor longus.
- Draw a straight line just posterior to the adductor longus tendon connecting the pubic tubercle to the medial femoral condyle — this approximates the anterior border of gracilis.
- Pedicle / perforator localization (practical marking sequence)
- From the pubic tubercle measure inferiorly ~8–12 cm and apply a handheld Doppler sweep here. The dominant pedicle / large musculocutaneous perforators are typically found in this zone (commonly cited 8–10 cm or 8–12 cm).
- Mark the strongest Doppler signal(s) with an “X” — plan your skin paddle to include this X.
- For TUG/TMG (transverse) design: center the transverse skin paddle over the upper third of the muscle, with the proximal limb ~4 cm inferior to the gluteal–groin crease. Transverse paddle can be up to ~30 × 10 cm in select patients but plan with a pinch test for primary closure.
- For longitudinal (vertical) design: set the proximal incision 2–3 cm below the pubic tubercle. The reliable skin paddle should remain within the proximal two‑thirds of the muscle (width typically 5–8 cm; length up to ~15 cm depending on laxity).
- Practical “pinch and Doppler” rule
- Use pinch test to ensure donor-site can be closed primarily. Usually up to ~9 cm width closes primarily.
- Always center the skin island over the Doppler-identified perforator(s). Perforators are most abundant in the proximal third; distal two‑thirds are less reliable.
- Muscle-specific markings for harvest or functional transfer
- Mark the full length of gracilis if needed (muscle length ≈ 25–30 cm; width ≈ 5 cm).
- If planning functional transfer, mark the muscle at set intervals (common teaching: 5‑cm markings along the muscle) while the muscle is at its in‑situ resting or stretched length — this preserves length–tension relationships at inset.
- Pitfalls related to markings
- Misidentifying adductor longus or sartorius → misplacing skin paddle or incision. Remember: gracilis lies posterior to adductor longus and is the only adductor crossing knee and hip (palpable on knee extension).
- Designing skin paddle into distal third of muscle → high risk of distal skin necrosis. Limit skin paddle to proximal two‑thirds unless delay or special technique used.
- Failing to Doppler/pincht — skin paddle not over perforator → unreliable cutaneous perfusion.
- Pearls and Pitfalls
- Mark saphenous vein course and avoid injuring it during anterior approach.
- For best results in free transfer, plan to dissect the pedicle proximally to the profunda/proximal source (gives extra 1–2 cm of reach and larger vessel caliber).
- For transverse TUG/TMG flap, orient the paddle posteriorly (captures more abundant posterior angiosome) and maintain a small anterior window over femoral triangle to avoid lymphatic injury.
- Always perform pinch test to ensure donor skin can be closed primarily (skin paddle width generally ≤9 cm for primary closure).
- Center the skin paddle over muscle; include the fascia and septum toward adductor longus to capture posterior perforators.
- When planning a TUG, keep the proximal limb ~4 cm below the gluteal–groin crease to avoid dog‑ears.
- Designing a skin paddle extending into the distal third of the muscle has a high risk distal tip necrosis.
- Skin paddle vertical length should not exceed ~2/3 muscle length; width limited by pinch test (≤9 cm commonly for tension‑free closure).
### b) Discuss the vascular classification, vessel size and the path/location of the describe dominant (medial femoral circumflex) and minor pedicles and typical entry point (8–12 cm inferior to pubic tubercle),
- Vascular classification
- Mathes–Nahai type II muscle: one dominant pedicle + multiple minor segmental pedicles.
- Dominant pedicle = branch of the medial femoral circumflex system (most common regional source: profunda femoris / deep femoral). In ≈10% the main pedicle may arise directly from the profunda femoris.
- Dominant pedicle: anatomy & path
- Entry point: enters deep (medial) surface of gracilis ~8–12 cm (commonly 8–10 cm) inferior to the pubic tubercle.
- The pedicle is very reliable and passes deep to (posterior to) the adductor longus and superficial to adductor magnus; the anterior branch of the obturator nerve usually enters the muscle ~1–2 cm proximal to vessels.
- Dominant pedicle: vessel size & length
- Arterial diameter: mean ≈ 1–1.6 mm (ranges reported 0.5–2.0 mm depending on series).
- Venous diameter (venae comitantes): mean ≈ 1.5–3 mm (often coalesce to a single larger vein near origin — useful for microanastomosis).
- Pedicle length: commonly reported 6–8 cm (can gain more length by dissecting to profunda femoris).
- Minor pedicles: anatomy & clinical notes
- Proximal minor pedicle: present in ~85% as additional supply; origins variable — small branch of obturator, medial circumflex, or femoral system. Ligation usually tolerated.
- Distal minor pedicles: multiple segmental vessels supplying mid/distal muscle — typically from superficial femoral artery; most distal can come from popliteal. These are small (diameter ~0.5 mm; length 2–3 cm).
- Septocutaneous perforator: often the first minor pedicle distal to the dominant pedicle — can supply skin; basis for harvesting small perforator flaps without muscle.
- Perforator distribution & angiosome
- Most musculocutaneous perforators concentrated in proximal two‑thirds, highest density in proximal third.
- Practical surgical implications
- Free tissue transfer: dissect pedicle to its source (profunda/proximal medial circumflex) for maximum vessel diameter and length to facilitate microanastomosis — sometimes necessary because artery can be <1 mm at origin.
- Extended dissection (beneath adductor longus) can add ~1–2 cm reach and often yields a larger single venous outflow (vena comitans confluence).
- If pedicle length/diameter insufficient, consider taking saphenous vein as secondary venous outflow or revise recipient selection.
- Avoid deep skeletonizing dissection around femoral triangle to minimize lymphedema risk — particularly with transverse designs.